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Hampton Research Corp crystal screen ht
Crystal Screen Ht, supplied by Hampton Research Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crystal+screen+ht/crystal+ht+screen/10__1107_slash_s2059798326005498-92-44-58
Average 86 stars, based on 1 article reviews
crystal screen ht - by Bioz Stars, 2026-10
86/100 stars

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Crystallization Assay:

Article Title: Impact of 2′-deoxyribo-purine substrates on nonenzymatic RNA template-directed primer extension
Article Snippet: 0.33 mM self-complementary 16-mer RNA sequences in nuclease-free water (Invitrogen, Waltham, MA) were heated up to 90°C for 2 min and then slowly cooled to room temperature. .. Crystal Screen HT, Index HT, Natrix HT (Hampton Research, Aliso Viejo, CA) and Nuc-Pro HTS (Jena Bioscience, Jena, Germany) kits were used to screen crystallization conditions at 20°C using the sitting-drop vapor diffusion method. .. An NT8 robotic system and Rock Imager (Formulatrix, Waltham, MA) were used for crystallization screening and for monitoring the crystal growth process.

Article Title: Combined structural and FMO-based insights into shaft pilin polymerization mechanism in Streptococcus sanguinis.
Article Snippet: Bacterial pili are proteinaceous polymers that facilitate diverse biological functions.. The SK36 strain of oral commensal bacterium Streptococcus sanguinis harbors sortase-assembled pili consisting of four proteins; PilA, PilB, PilC, and PilX.. However, details regarding their structures and assembly mechanisms remain unclear.

Article Title: Structure of the <i>Arabidopsis</i> receptor kinase SRF6 ectodomain determined from crystals obtained using the LRR crystallization screen
Article Snippet: .. This was based on the idea that extracellular proteins usually reside in an acidic environment (the pH range of the plant apoplast is 4.5–6.5; Almeida & Huber, 1999) and that the mean pH of many commercially available, high-throughput crystallization screens is close to neutral (Crystal Screen HT, pH 6.6; Index HT, mean pH 6.8; PEG Ion, pH 6.8; Hampton Research). ..

Article Title: Identification, functional characterization, and structural analysis of an atypical l -threonate 3-dehydrogenase
Article Snippet: The purified enzyme was loaded onto a HiLoad 16/600 Superdex 200 pg column equilibrated with buffer D. A high–molecular-weight gel filtration calibration kit (GE Healthcare) was used with the following molecular markers: thyroglobulin, 669 kDa; ferritin, 440 kDa; aldolase, 158 kDa; conalbumin, 75 kDa; and ovalbumin, 44 kDa. .. Crystallization of the purified Pl Ltn3D protein was initially performed with Index and Crystal Screen HT (Hampton Research) using the sitting-drop vapor diffusion method at 20 °C. ..

Diffusion-based Assay:

Article Title: Impact of 2′-deoxyribo-purine substrates on nonenzymatic RNA template-directed primer extension
Article Snippet: 0.33 mM self-complementary 16-mer RNA sequences in nuclease-free water (Invitrogen, Waltham, MA) were heated up to 90°C for 2 min and then slowly cooled to room temperature. .. Crystal Screen HT, Index HT, Natrix HT (Hampton Research, Aliso Viejo, CA) and Nuc-Pro HTS (Jena Bioscience, Jena, Germany) kits were used to screen crystallization conditions at 20°C using the sitting-drop vapor diffusion method. .. An NT8 robotic system and Rock Imager (Formulatrix, Waltham, MA) were used for crystallization screening and for monitoring the crystal growth process.

Article Title: Combined structural and FMO-based insights into shaft pilin polymerization mechanism in Streptococcus sanguinis.
Article Snippet: Bacterial pili are proteinaceous polymers that facilitate diverse biological functions.. The SK36 strain of oral commensal bacterium Streptococcus sanguinis harbors sortase-assembled pili consisting of four proteins; PilA, PilB, PilC, and PilX.. However, details regarding their structures and assembly mechanisms remain unclear.

Article Title: Identification, functional characterization, and structural analysis of an atypical l -threonate 3-dehydrogenase
Article Snippet: The purified enzyme was loaded onto a HiLoad 16/600 Superdex 200 pg column equilibrated with buffer D. A high–molecular-weight gel filtration calibration kit (GE Healthcare) was used with the following molecular markers: thyroglobulin, 669 kDa; ferritin, 440 kDa; aldolase, 158 kDa; conalbumin, 75 kDa; and ovalbumin, 44 kDa. .. Crystallization of the purified Pl Ltn3D protein was initially performed with Index and Crystal Screen HT (Hampton Research) using the sitting-drop vapor diffusion method at 20 °C. ..

Article Title: Methods of eliciting antibodies that bind to full-length glycosylated HIV-1 ENV using multimerized ENV cores
Article Snippet: Crystallization conditions were screened and monitored with an NT8 drop setter and Rock Imager (Formulatrix). .. Screening was done with Rigaku Wizard Precipitant Synergy block no. 2 (MD15-PS-B), Molecular Dimensions Proplex screen HT-96 (MD1-38), and Hampton Research Crystal Screen HT (HR2-130) using the sitting drop vapor diffusion method. ..

Purification:

Article Title: Identification, functional characterization, and structural analysis of an atypical l -threonate 3-dehydrogenase
Article Snippet: The purified enzyme was loaded onto a HiLoad 16/600 Superdex 200 pg column equilibrated with buffer D. A high–molecular-weight gel filtration calibration kit (GE Healthcare) was used with the following molecular markers: thyroglobulin, 669 kDa; ferritin, 440 kDa; aldolase, 158 kDa; conalbumin, 75 kDa; and ovalbumin, 44 kDa. .. Crystallization of the purified Pl Ltn3D protein was initially performed with Index and Crystal Screen HT (Hampton Research) using the sitting-drop vapor diffusion method at 20 °C. ..

Blocking Assay:

Article Title: Methods of eliciting antibodies that bind to full-length glycosylated HIV-1 ENV using multimerized ENV cores
Article Snippet: Crystallization conditions were screened and monitored with an NT8 drop setter and Rock Imager (Formulatrix). .. Screening was done with Rigaku Wizard Precipitant Synergy block no. 2 (MD15-PS-B), Molecular Dimensions Proplex screen HT-96 (MD1-38), and Hampton Research Crystal Screen HT (HR2-130) using the sitting drop vapor diffusion method. ..



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